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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411
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        This report was generated using MultiQC, version 1.28

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2025-04-02, 14:07 EDT based on data in:
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        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_DCRW_A1_S65_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_DCRW_A1_S65_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_DCRW_B1_S28_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_DCRW_B1_S28_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_FieldBlank_1_S66_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_FieldBlank_1_S66_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_FieldBlank_2_S15_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_FieldBlank_2_S15_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRL_A1_S114_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRL_A1_S114_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRL_B1_S51_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRL_B1_S51_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRL_B2_S30_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRL_B2_S30_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRW_A1_S104_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRW_A1_S104_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRW_A2_S48_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRW_A2_S48_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRW_B1_S10_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HINRW_B1_S10_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRL_A1_S153_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRL_A1_S153_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRL_A2_S54_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRL_A2_S54_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRL_B1_S3_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRL_B1_S3_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRW_A1_S42_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRW_A1_S42_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRW_A2_S106_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRW_A2_S106_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRW_B1_S25_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRW_B1_S25_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRW_B2_S7_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_HIRW_B2_S7_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_LabBlank_1_S49_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_LabBlank_1_S49_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TANRL_A1_S100_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TANRL_A1_S100_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TANRW_A1_S14_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TANRW_A1_S14_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TANRW_B1_S117_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TANRW_B1_S117_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARL_A1_S22_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARL_A1_S22_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARL_B1_S4_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARL_B1_S4_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARL_B2_S63_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARL_B2_S63_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARW_A1_S39_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARW_A1_S39_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARW_B1_S52_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Nov_TARW_B1_S52_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Sep_ExtractionBlank_5_S12_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/23-Sep_ExtractionBlank_5_S12_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/24-Sep_ExtractionBlank_6_S146_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/24-Sep_ExtractionBlank_6_S146_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/27-Jan_ExtractionBlank_7_S141_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/27-Jan_ExtractionBlank_7_S141_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/28-Jan_ExtractionBlank_8_S154_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/28-Jan_ExtractionBlank_8_S154_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_E_EB_S131_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_E_EB_S131_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_E_S19_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_E_S19_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_I_EB_S68_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_I_EB_S68_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_I_S77_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_I_S77_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_L_EB_S13_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_L_EB_S13_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_N_EB_S82_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_N_EB_S82_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_N_S61_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_N_S61_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_T_EB_S109_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_T_EB_S109_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_T_S149_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/29-Sep_PR_T_S149_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_1_S31_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_1_S31_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_2_S62_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_2_S62_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_3_S93_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_3_S93_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_4_S124_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_4_S124_R1_001_fastqc.zip
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_5_S155_R1_001_fastqc.html
        • /Volumes/Fuji/Mangroves/2025_0319_Givens_Canty_Rookery_COI/cutadapt/trim/reports-fastqc/PCR_Blank_5_S155_R1_001_fastqc.zip

        Because this report contains a lot of samples, you may need to click 'Show plot' to see some graphs.

        General Statistics

        Showing 155/155 rows and 3/6 columns.
        Sample NameDupsGCAvg lenMedian lenFailedSeqs
        15-Aug_DCNRL_A1_S29_R1_001
        96.3%
        38.0%
        274bp
        274bp
        45%
        0.5M
        15-Aug_DCNRL_B1_S71_R1_001
        96.2%
        38.0%
        274bp
        274bp
        45%
        0.4M
        15-Aug_DCNRL_B2_S140_R1_001
        97.0%
        38.0%
        274bp
        274bp
        45%
        0.6M
        15-Aug_DCNRW_A1_S44_R1_001
        96.5%
        38.0%
        274bp
        274bp
        45%
        0.4M
        15-Aug_DCNRW_B1_S6_R1_001
        97.1%
        38.0%
        274bp
        274bp
        45%
        0.6M
        15-Aug_DCRL_A1_S99_R1_001
        96.8%
        36.0%
        274bp
        274bp
        45%
        0.9M
        15-Aug_DCRL_A2_S55_R1_001
        96.1%
        36.0%
        274bp
        274bp
        45%
        0.5M
        15-Aug_DCRL_B1_S24_R1_001
        94.7%
        36.0%
        274bp
        274bp
        45%
        0.2M
        15-Aug_DCRL_B2_S122_R1_001
        97.2%
        36.0%
        274bp
        274bp
        45%
        1.1M
        15-Aug_DCRW_A1_S102_R1_001
        95.9%
        36.0%
        274bp
        274bp
        45%
        0.5M
        15-Aug_DCRW_A2_S127_R1_001
        95.8%
        37.0%
        274bp
        274bp
        45%
        0.7M
        15-Aug_DCRW_B1_S74_R1_001
        96.2%
        36.0%
        274bp
        274bp
        45%
        0.6M
        15-Aug_DCRW_B2_S2_R1_001
        96.5%
        36.0%
        274bp
        274bp
        45%
        0.6M
        15-Aug_FieldBlank_1_S43_R1_001
        79.7%
        38.0%
        274bp
        274bp
        45%
        0.0M
        15-Aug_HINRL_A1_S59_R1_001
        97.2%
        38.0%
        274bp
        274bp
        45%
        0.5M
        15-Aug_HINRL_B1_S53_R1_001
        95.4%
        37.0%
        274bp
        274bp
        45%
        0.3M
        15-Aug_HINRL_B2_S98_R1_001
        97.5%
        38.0%
        274bp
        274bp
        45%
        0.8M
        15-Aug_HINRW_A1_S18_R1_001
        95.7%
        39.0%
        274bp
        274bp
        45%
        0.2M
        15-Aug_HINRW_A2_S69_R1_001
        97.3%
        39.0%
        274bp
        274bp
        45%
        0.5M
        15-Aug_HINRW_B1_S83_R1_001
        97.0%
        37.0%
        274bp
        274bp
        45%
        0.5M
        15-Aug_HIRL_A1_S129_R1_001
        96.4%
        38.0%
        274bp
        274bp
        45%
        0.7M
        15-Aug_HIRL_B1_S41_R1_001
        96.1%
        38.0%
        274bp
        274bp
        36%
        0.5M
        15-Aug_HIRL_B2_S1_R1_001
        97.1%
        38.0%
        274bp
        274bp
        45%
        0.6M
        15-Aug_HIRW_A1_S73_R1_001
        94.9%
        37.0%
        274bp
        274bp
        45%
        0.5M
        15-Aug_HIRW_A2_S27_R1_001
        95.5%
        37.0%
        274bp
        274bp
        45%
        0.5M
        15-Aug_HIRW_B1_S151_R1_001
        95.0%
        37.0%
        274bp
        274bp
        45%
        0.4M
        15-Aug_HIRW_B2_S26_R1_001
        94.9%
        37.0%
        274bp
        274bp
        45%
        0.4M
        15-Aug_LabBlank_S105_R1_001
        78.0%
        38.0%
        274bp
        274bp
        45%
        0.0M
        16-Sep_ExtractionBlank_1_S5_R1_001
        85.0%
        38.0%
        274bp
        274bp
        45%
        0.0M
        17-Aug_FieldBlank_2_S134_R1_001
        76.1%
        39.0%
        274bp
        274bp
        45%
        0.0M
        17-Aug_LabBlank_S97_R1_001
        81.3%
        38.0%
        274bp
        274bp
        45%
        0.0M
        17-Aug_TANRL_A1_S150_R1_001
        90.3%
        40.0%
        274bp
        274bp
        45%
        0.1M
        17-Aug_TANRL_A2_S132_R1_001
        91.1%
        42.0%
        274bp
        274bp
        45%
        0.2M
        17-Aug_TANRL_B1_S147_R1_001
        93.8%
        39.0%
        274bp
        274bp
        45%
        0.1M
        17-Aug_TANRL_B2_S38_R1_001
        83.3%
        48.0%
        271bp
        274bp
        55%
        0.1M
        17-Aug_TANRW_A1_S46_R1_001
        95.1%
        39.0%
        274bp
        274bp
        45%
        0.2M
        17-Aug_TANRW_B1_S70_R1_001
        94.9%
        39.0%
        274bp
        274bp
        45%
        0.2M
        17-Aug_TARL_A1_S125_R1_001
        94.8%
        38.0%
        274bp
        274bp
        45%
        0.5M
        17-Aug_TARL_B1_S136_R1_001
        94.7%
        38.0%
        274bp
        274bp
        45%
        0.3M
        17-Aug_TARW_A1_S21_R1_001
        89.8%
        38.0%
        274bp
        274bp
        45%
        0.0M
        17-Aug_TARW_B1_S85_R1_001
        94.6%
        38.0%
        274bp
        274bp
        45%
        0.2M
        17-Sep_ExtractionBlank_2_S50_R1_001
        95.5%
        36.0%
        274bp
        274bp
        45%
        0.4M
        18-Sep_ExtractionBlank_3_S60_R1_001
        80.2%
        38.0%
        274bp
        274bp
        45%
        0.0M
        21-Sep_ExtractionBlank_4_S23_R1_001
        70.8%
        39.0%
        274bp
        274bp
        45%
        0.0M
        22-Feb_DCNRL_A1_S45_R1_001
        96.1%
        38.0%
        274bp
        274bp
        45%
        0.4M
        22-Feb_DCNRL_B1_S113_R1_001
        96.7%
        38.0%
        274bp
        274bp
        45%
        0.6M
        22-Feb_DCNRW_A1_S96_R1_001
        96.9%
        38.0%
        274bp
        274bp
        45%
        0.8M
        22-Feb_DCNRW_A2_S121_R1_001
        97.5%
        37.0%
        274bp
        274bp
        45%
        0.9M
        22-Feb_DCNRW_B1_S137_R1_001
        96.5%
        39.0%
        274bp
        274bp
        45%
        0.5M
        22-Feb_DCRL_A1_S64_R1_001
        96.3%
        39.0%
        274bp
        274bp
        45%
        0.4M
        22-Feb_DCRL_B1_S35_R1_001
        96.7%
        38.0%
        274bp
        274bp
        45%
        0.6M
        22-Feb_DCRW_A1_S47_R1_001
        95.4%
        39.0%
        274bp
        274bp
        45%
        0.6M
        22-Feb_DCRW_B1_S130_R1_001
        95.7%
        39.0%
        274bp
        274bp
        45%
        0.5M
        22-Feb_DCRW_B2_S111_R1_001
        95.0%
        39.0%
        274bp
        274bp
        45%
        0.5M
        22-Feb_FieldBlank_1_S120_R1_001
        83.6%
        38.0%
        274bp
        274bp
        45%
        0.0M
        22-Feb_TANRL_A1_S138_R1_001
        88.6%
        39.0%
        274bp
        274bp
        45%
        0.0M
        22-Feb_TANRL_B1_S40_R1_001
        79.0%
        44.0%
        273bp
        274bp
        55%
        0.0M
        22-Feb_TANRW_A1_S88_R1_001
        95.6%
        38.0%
        274bp
        274bp
        45%
        0.4M
        22-Feb_TANRW_B1_S116_R1_001
        93.8%
        37.0%
        274bp
        274bp
        45%
        0.1M
        22-Feb_TANRW_B2_S75_R1_001
        93.2%
        38.0%
        274bp
        274bp
        45%
        0.1M
        22-Feb_TARL_A1_S144_R1_001
        96.5%
        38.0%
        274bp
        274bp
        45%
        0.4M
        22-Feb_TARL_B1_S84_R1_001
        94.9%
        39.0%
        274bp
        274bp
        45%
        0.2M
        22-Feb_TARW_A1_S81_R1_001
        95.2%
        38.0%
        274bp
        274bp
        45%
        0.3M
        22-Feb_TARW_B1_S20_R1_001
        91.4%
        38.0%
        274bp
        274bp
        45%
        0.1M
        22-Feb_TARW_B2_S115_R1_001
        94.6%
        39.0%
        274bp
        274bp
        45%
        0.2M
        23-Feb_FieldBlank_2_S139_R1_001
        72.4%
        39.0%
        274bp
        274bp
        45%
        0.0M
        23-Feb_HINRL_A1_S112_R1_001
        97.0%
        39.0%
        274bp
        274bp
        45%
        0.6M
        23-Feb_HINRL_B1_S143_R1_001
        97.0%
        39.0%
        274bp
        274bp
        45%
        0.5M
        23-Feb_HINRW_A1_S78_R1_001
        96.9%
        39.0%
        274bp
        274bp
        45%
        0.5M
        23-Feb_HINRW_B1_S101_R1_001
        97.2%
        39.0%
        274bp
        274bp
        45%
        0.6M
        23-Feb_HIRL_A1_S8_R1_001
        97.0%
        38.0%
        274bp
        274bp
        45%
        0.6M
        23-Feb_HIRL_B1_S80_R1_001
        96.1%
        38.0%
        274bp
        274bp
        45%
        0.6M
        23-Feb_HIRW_A1_S148_R1_001
        97.3%
        39.0%
        274bp
        274bp
        45%
        0.7M
        23-Feb_HIRW_B1_S110_R1_001
        96.5%
        38.0%
        274bp
        274bp
        45%
        0.5M
        23-Feb_LabBlank_1_S126_R1_001
        78.5%
        39.0%
        274bp
        274bp
        45%
        0.0M
        23-May_DCNRL_A1_S145_R1_001
        97.1%
        38.0%
        274bp
        274bp
        45%
        0.8M
        23-May_DCNRL_B1_S76_R1_001
        96.6%
        38.0%
        274bp
        274bp
        45%
        0.6M
        23-May_DCNRW_A1_S67_R1_001
        96.3%
        38.0%
        274bp
        274bp
        45%
        0.4M
        23-May_DCNRW_B1_S36_R1_001
        96.5%
        38.0%
        274bp
        274bp
        45%
        0.5M
        23-May_DCRL_A1_S95_R1_001
        96.4%
        38.0%
        274bp
        274bp
        45%
        0.7M
        23-May_DCRL_B1_S119_R1_001
        95.6%
        38.0%
        274bp
        274bp
        45%
        0.6M
        23-May_DCRL_B2_S87_R1_001
        95.8%
        38.0%
        274bp
        274bp
        45%
        0.5M
        23-May_DCRW_A1_S11_R1_001
        96.0%
        37.0%
        274bp
        274bp
        45%
        0.4M
        23-May_DCRW_B1_S128_R1_001
        95.3%
        36.0%
        274bp
        274bp
        45%
        0.8M
        23-May_FieldBlank_1_S79_R1_001
        81.6%
        39.0%
        274bp
        274bp
        45%
        0.0M
        23-May_FieldBlank_2_S107_R1_001
        75.8%
        38.0%
        274bp
        274bp
        45%
        0.0M
        23-May_HINRL_A1_S34_R1_001
        96.8%
        39.0%
        274bp
        274bp
        45%
        0.6M
        23-May_HINRL_B1_S57_R1_001
        97.0%
        39.0%
        274bp
        274bp
        45%
        0.5M
        23-May_HINRW_A1_S9_R1_001
        97.0%
        39.0%
        274bp
        274bp
        45%
        0.6M
        23-May_HINRW_B1_S142_R1_001
        97.0%
        39.0%
        274bp
        274bp
        45%
        0.6M
        23-May_HINRW_B2_S90_R1_001
        93.7%
        37.0%
        274bp
        274bp
        45%
        0.3M
        23-May_HIRL_A1_S32_R1_001
        96.7%
        38.0%
        274bp
        274bp
        45%
        0.7M
        23-May_HIRL_B1_S33_R1_001
        96.5%
        38.0%
        274bp
        274bp
        45%
        0.6M
        23-May_HIRW_A1_S118_R1_001
        71.3%
        46.0%
        273bp
        274bp
        55%
        0.0M
        23-May_HIRW_B1_S86_R1_001
        96.8%
        39.0%
        274bp
        274bp
        45%
        0.5M
        23-May_HIRW_B2_S133_R1_001
        76.0%
        38.0%
        274bp
        274bp
        45%
        0.0M
        23-May_LabBlank_1_S37_R1_001
        80.6%
        40.0%
        274bp
        274bp
        45%
        0.0M
        23-May_TANRL_A1_S17_R1_001
        94.3%
        38.0%
        274bp
        274bp
        45%
        0.2M
        23-May_TANRL_B1_S108_R1_001
        96.6%
        37.0%
        274bp
        274bp
        45%
        0.4M
        23-May_TANRW_A1_S135_R1_001
        96.2%
        37.0%
        274bp
        274bp
        45%
        0.3M
        23-May_TANRW_B1_S92_R1_001
        97.1%
        36.0%
        274bp
        274bp
        45%
        0.6M
        23-May_TARL_A1_S72_R1_001
        95.6%
        37.0%
        274bp
        274bp
        45%
        0.5M
        23-May_TARL_B1_S103_R1_001
        95.7%
        38.0%
        274bp
        274bp
        45%
        0.4M
        23-May_TARW_A1_S94_R1_001
        96.1%
        38.0%
        274bp
        274bp
        45%
        0.6M
        23-May_TARW_B1_S89_R1_001
        95.5%
        38.0%
        274bp
        274bp
        45%
        0.5M
        23-Nov_DCNRL_A1_S58_R1_001
        95.9%
        37.0%
        274bp
        274bp
        45%
        0.5M
        23-Nov_DCNRL_B1_S56_R1_001
        96.2%
        37.0%
        274bp
        274bp
        45%
        0.7M
        23-Nov_DCNRW_A1_S16_R1_001
        96.4%
        37.0%
        274bp
        274bp
        45%
        0.6M
        23-Nov_DCNRW_B1_S123_R1_001
        96.7%
        37.0%
        274bp
        274bp
        45%
        0.8M
        23-Nov_DCRL_A1_S91_R1_001
        95.8%
        38.0%
        274bp
        274bp
        45%
        0.6M
        23-Nov_DCRL_B1_S152_R1_001
        96.0%
        38.0%
        274bp
        274bp
        45%
        0.8M
        23-Nov_DCRW_A1_S65_R1_001
        96.5%
        36.0%
        274bp
        274bp
        45%
        0.6M
        23-Nov_DCRW_B1_S28_R1_001
        96.9%
        36.0%
        274bp
        274bp
        45%
        0.7M
        23-Nov_FieldBlank_1_S66_R1_001
        82.4%
        38.0%
        274bp
        274bp
        45%
        0.0M
        23-Nov_FieldBlank_2_S15_R1_001
        75.5%
        40.0%
        274bp
        274bp
        45%
        0.0M
        23-Nov_HINRL_A1_S114_R1_001
        96.6%
        38.0%
        274bp
        274bp
        45%
        0.7M
        23-Nov_HINRL_B1_S51_R1_001
        97.4%
        39.0%
        274bp
        274bp
        45%
        0.5M
        23-Nov_HINRL_B2_S30_R1_001
        96.7%
        37.0%
        274bp
        274bp
        45%
        0.6M
        23-Nov_HINRW_A1_S104_R1_001
        96.1%
        37.0%
        274bp
        274bp
        45%
        0.5M
        23-Nov_HINRW_A2_S48_R1_001
        96.8%
        37.0%
        274bp
        274bp
        45%
        0.5M
        23-Nov_HINRW_B1_S10_R1_001
        94.4%
        40.0%
        274bp
        274bp
        45%
        0.2M
        23-Nov_HIRL_A1_S153_R1_001
        98.3%
        37.0%
        274bp
        274bp
        45%
        2.7M
        23-Nov_HIRL_A2_S54_R1_001
        67.5%
        49.0%
        271bp
        274bp
        55%
        0.0M
        23-Nov_HIRL_B1_S3_R1_001
        96.6%
        37.0%
        274bp
        274bp
        45%
        0.6M
        23-Nov_HIRW_A1_S42_R1_001
        94.9%
        37.0%
        274bp
        274bp
        45%
        0.4M
        23-Nov_HIRW_A2_S106_R1_001
        95.7%
        37.0%
        274bp
        274bp
        45%
        0.5M
        23-Nov_HIRW_B1_S25_R1_001
        95.8%
        37.0%
        274bp
        274bp
        45%
        0.6M
        23-Nov_HIRW_B2_S7_R1_001
        97.2%
        37.0%
        274bp
        274bp
        45%
        0.7M
        23-Nov_LabBlank_1_S49_R1_001
        82.1%
        38.0%
        274bp
        274bp
        45%
        0.0M
        23-Nov_TANRL_A1_S100_R1_001
        95.8%
        38.0%
        274bp
        274bp
        45%
        0.2M
        23-Nov_TANRW_A1_S14_R1_001
        95.8%
        38.0%
        274bp
        274bp
        45%
        0.3M
        23-Nov_TANRW_B1_S117_R1_001
        95.9%
        37.0%
        274bp
        274bp
        45%
        0.4M
        23-Nov_TARL_A1_S22_R1_001
        94.9%
        38.0%
        274bp
        274bp
        45%
        0.2M
        23-Nov_TARL_B1_S4_R1_001
        96.2%
        38.0%
        274bp
        274bp
        45%
        0.4M
        23-Nov_TARL_B2_S63_R1_001
        86.4%
        38.0%
        274bp
        274bp
        45%
        0.0M
        23-Nov_TARW_A1_S39_R1_001
        96.3%
        37.0%
        274bp
        274bp
        45%
        0.5M
        23-Nov_TARW_B1_S52_R1_001
        96.7%
        38.0%
        274bp
        274bp
        45%
        0.4M
        23-Sep_ExtractionBlank_5_S12_R1_001
        80.9%
        38.0%
        274bp
        274bp
        45%
        0.0M
        24-Sep_ExtractionBlank_6_S146_R1_001
        81.6%
        38.0%
        274bp
        274bp
        45%
        0.0M
        27-Jan_ExtractionBlank_7_S141_R1_001
        80.9%
        38.0%
        274bp
        274bp
        45%
        0.0M
        28-Jan_ExtractionBlank_8_S154_R1_001
        80.7%
        38.0%
        274bp
        274bp
        45%
        0.0M
        29-Sep_PR_E_EB_S131_R1_001
        74.4%
        39.0%
        274bp
        274bp
        45%
        0.0M
        29-Sep_PR_E_S19_R1_001
        94.7%
        38.0%
        274bp
        274bp
        45%
        0.2M
        29-Sep_PR_I_EB_S68_R1_001
        83.6%
        38.0%
        274bp
        274bp
        45%
        0.0M
        29-Sep_PR_I_S77_R1_001
        96.7%
        38.0%
        274bp
        274bp
        45%
        0.5M
        29-Sep_PR_L_EB_S13_R1_001
        81.8%
        38.0%
        274bp
        274bp
        45%
        0.0M
        29-Sep_PR_N_EB_S82_R1_001
        81.1%
        38.0%
        274bp
        274bp
        45%
        0.0M
        29-Sep_PR_N_S61_R1_001
        96.7%
        37.0%
        274bp
        274bp
        45%
        0.6M
        29-Sep_PR_T_EB_S109_R1_001
        75.1%
        39.0%
        274bp
        274bp
        45%
        0.0M
        29-Sep_PR_T_S149_R1_001
        96.2%
        38.0%
        274bp
        274bp
        45%
        0.6M
        PCR_Blank_1_S31_R1_001
        81.4%
        38.0%
        274bp
        274bp
        45%
        0.0M
        PCR_Blank_2_S62_R1_001
        79.8%
        38.0%
        274bp
        274bp
        45%
        0.0M
        PCR_Blank_3_S93_R1_001
        77.4%
        38.0%
        274bp
        274bp
        45%
        0.0M
        PCR_Blank_4_S124_R1_001
        79.9%
        39.0%
        274bp
        274bp
        45%
        0.0M
        PCR_Blank_5_S155_R1_001
        81.5%
        38.0%
        274bp
        274bp
        45%
        0.0M

        FastQC

        Version: 0.12.1

        Quality control tool for high throughput sequencing data.URL: http://www.bioinformatics.babraham.ac.uk/projects/fastqc

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Created with MultiQC

        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Created with MultiQC

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Created with MultiQC

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Created with MultiQC

        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Created with MultiQC

        Sequence Length Distribution

        The distribution of fragment sizes (read lengths) found. See the FastQC help

        Created with MultiQC

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (e.g. PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Created with MultiQC

        Overrepresented sequences by sample

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as overrepresented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Created with MultiQC

        Top overrepresented sequences

        Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

        Showing 20/20 rows and 3/3 columns.
        Overrepresented sequenceReportsOccurrences% of all reads
        GTTATCATCGACTGCTGGTCAACCTGGTCCTGCAATGGATTTAGCAATTT
        154
        4142740
        6.8337%
        TTTAAGTTCCATTACTGGGCATAGCTCACCCGCTGTAGATATGGCTATTT
        152
        2976785
        4.9104%
        GCTTTCTACAACAGCTCAACCAGGTCCAGCGATGGATTTAGCGATATTAA
        151
        639935
        1.0556%
        GCTTTCTACAACAGCTCAACCAGGTCCTGCGATGGATTTAGCGATATTAA
        151
        637683
        1.0519%
        TTTAAGTTCCATTACTGGACATAGCTCACCCGCTGTAGATATGGCTATTT
        150
        3299884
        5.4433%
        GTTATCATCGACCGCTGGTCAACCTGGTCCTGCAATGGATTTAGCAATTT
        150
        570113
        0.9404%
        TTTGAGTTCGATTACTGGCCATAGCTCTCCCGCAGTAGATATGGCTATTT
        149
        972619
        1.6044%
        TTTAAGAGGAAATGTTTCTCATAGGGGGGCGGCGGTTGACTTCGCTATTT
        148
        2268773
        3.7425%
        ATTAAGTGCCTTAGAAAAGGCCATGCCAGGTTCTGGTTTGGGTATGACTT
        148
        1275066
        2.1033%
        GCTTTCTACAACTGCTCAACCAGGTCCTGCGATGGATTTAGCGATATTAA
        143
        403316
        0.6653%
        TTTATCAGGTATAGAAGCCCATTCAGGTGCGTCTGTAGATTTAGCAATTT
        142
        269744
        0.4450%
        GTTGAGTTCGATTACTGGCCATAGCTCTCCTGCAGTAGATATGGCTATTT
        140
        1487558
        2.4538%
        ATTGAGTGCCTTACCTCAAGCCCAACCCGGTTCTGGTTTGGGGATGACGC
        138
        335026
        0.5526%
        TTTGAGTTCGATTACTGGCCATAGTTCTCCTGCAGTAGATATGGCTATTT
        137
        548237
        0.9043%
        TTTGAGTTCGATCACTGGCCATAGCTCTCCTGCAGTAGATATGGCTATTT
        136
        466507
        0.7695%
        ATTGTCAAGTGCAACAGCACATTCTGGAGGTTCCGTAGATTTAGCCATTT
        131
        312522
        0.5155%
        ATTATCATCAACTGCTGGTCAACCAGGCCCTGCAATGGACTTAGCAATTT
        129
        356862
        0.5887%
        GCTTTCTACAACAGCTCAACCAGGTCCAGCGATGGATTTAGCAATATTAA
        125
        142443
        0.2350%
        ACTTAGTGCATTACCTCAGGCACAGCCTGGATCTGCCGCTGGTATGACTT
        123
        928067
        1.5309%
        ATTATCATCAACTGCTGGTCAACCAGGTCCTGCAATGGATTTAGCAATTT
        123
        174390
        0.2877%

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Created with MultiQC

        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

        Created with MultiQC

        Software Versions

        Software Versions lists versions of software tools extracted from file contents.

        SoftwareVersion
        FastQC0.12.1